This topical collection presents peer-reviewed scientific results from the SELINA project, funded by Horizon Europe. The collection brings together applied research focused on innovative methods, practical case studies, and policy-relevant insights that support the uptake of nature-based knowledge in environmental management, spatial planning, and socio-economic development. Serving as an open-access resource, the collection aims to foster interdisciplinary dialogue and enhance the visibility and usability of SELINA’s findings among scientists, practitioners, and policymakers.